Eukaryotic Gene Expression Transcription |
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Prokaryotes have a single RNA polymerase which is responsible for all transcription.
Eukaryotes have three distinct RNA polymerases, each transcribing a distinct set of RNAs. |
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RNA Polymerase I
RNA Polymerase II RNA Polymerase III |
rRNAs
mRNA precursors (hn-RNAs) small functional RNAs |
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We will focus the rest of our discussion on RNA Polymerase II as this enzyme is responsible for the synthesis of protein encoding mRNAs...
The process of transcription begins with promoter identification and the initiation of transcription. In prokaryotes, this is achieved by the interaction of In eukaryotes, the process of promoter selection is much more complex... Like prokaryotes, eukaryotic genes have a promoter sequence located upstream of the initiation of txn |
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This TATA box is the target sequence for a sequence specific DNA binding protein called TATA-Binding Protein (TBP) |
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TBP binds to the (-35) TATA box and acts as a nucleation center for the recruitment of TBP-Associated-Factors (TAFs) |
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Protein-protein interactions between TBP and the TAFs (TF II ___) produces a basal transcription complex |
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Index |
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