Thompson Rivers University Department of Biological Sciences Thompson Rivers University Depart of Biological Sciences

Biocatalyst and Bioproduct Development Facility (BBDF)

Current Research:

Research in the BBDF has focussed on the biodegradation of organic contaminants, mainly sulfur-containing and halogenated compounds. More recently, we have been interested in microbial communities of interest in agricultural settings, including development of feed additives to reduce methane production in cattle.

Culturing and Fermentation:

The lab is equipped to carry out fermentation optimization studies and the production of up to 40 L of bacterial, yeast, fungal or mammalian cell cultures for a wide range of applications in the environmental, chemical and pharmaceutical fields. Downstream processing includes centrifugation, homogenization and cross-flow filtration.

14-L Stirred Tank Reactors

Available Equiment:

Proteomics and Protein Purification:

The BBDF houses equipment for 2D DIGE is available for proteomic studies, and includes a fluorescence imager and robotic spot picker. A state of the art protein purification system is also available.

Protein Purification System
Ettan Robotic Spot Picker
Typhoon Imager

Available Equiment:

Molecular Biology and Sequencing:

The BBDF has equipment for traditional molecular biology and molecular microbial ecology, including PCR and real time PCR, Denaturing Gradient Gel Electrophoresis (DGGE) and Repetitive Sequence-Based PCR (rep-PCR). TRU also has an IonTorrent Personal Genome Machine (PGM) Genetic Sequencer that can be made fully accessible to visiting scientists.

IonTorrent Gene Sequencer
PCR Thermo-Cycler
PCR and Gel Electrophoresis
More Information About The IonTorrent PGM Genetic Squencer

Analytical Equipment:

The BBDF houses a gas chromatograph with Flame Ionization Detector, Electron Capture Detector and Pulsed Flame Photometric Detector, as well as a CombiPAL autosampler for liquid, headspace and Solid Phase Micro Extraction injections.

TRU Also Has the Following Analytical Equiment: